This user manual explains how to acquire image data on an ivis imaging system and analyze images using the living image software. Please contact your local support group before installing this update. Wsu has acquired an ivis spectrum ct by caliper instruments, a perkinelmer company that is available to the general wsu community. Like previous ivis systems, it uses xenogens novel patented optical imaging technology to facilitate noninvasive longitudinal monitoring of disease progression, cell trafficking, and. Perkinelmer has led the way for over 25 years with powerful software for its in vivo imaging systems. The seamless, end to end workflow from acquisition to analysis maps user journeys through the most complex protocols, providing breakthrough productivity. Ivis in vivo imaging system is a novel technology that enables one to measure optical probes bioluminescence, fluorescence, and cerenkov imaging for radiotracers in live animals. Instrumentation resource facility school of medicine. Whether your concerns are compliance, planning, work control, cost tracking, or quality management, ivis handles it. Noninvasive in vivo imaging allows researchers to sequentially image animals over time yielding valuable information on disease progression, cell trafficking and gene expression patterns in living animals.
The system uses leading optical technology for preclinical imaging research and development ideal for noninvasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals. Looking for online definition of ivis or what ivis stands for. If you have questions regarding this manual or the ivis imaging system, please call ivis imaging systems technology support. Perkinelmer caliper ivis spectrum hardware manual pdf download. This user manual explains how to acquire optical and volumetric image data on the ivis spectrum and analyze the data using the living image software. Started in 2002 and supported by some of the best winemakers in the u. The ivis spectrum is a bioluminescence and fluorescence in vivo imaging system, which allows for noninvasive tracking and measurement of disease progression, cellular activity and gene expression.
In vivo bioluminescent imaging of mammary tumors using. This user manual explains how to acquire optical image data on an ivis imaging system and analyze images using the living image software. Perkinelmer ivis spectrum fluorescence and bioluminescence imaging system. Bioluminescent imager for measuring and quantifying fluorescent or chemiluminescent luciferinluciferase signal expression in. The living image software automatically coregisters the photographic and bioluminescence images to generate the overlay image. Filters and spectral unmixing algorithms let you take full advantage of bioluminescent and fluorescent reporters across the blue to near infrared wavelength region. Living image advanced in vivo imaging software designed for the ivis. The living image software controls the ivis spectrum, and displays and analyzes the image data. Caliper life sciences living image software automates all the controls and settings required for seamless image acquisition and processing. The rtsf ivis spectrum imaging core at michigan state university is a versatile, advanced in vivo bioluminescent and fluorescent preclinical imaging system that. This training video discusses using the ivis platform for in vivo bioluminescence imaging studies to evaluate 2 separate mouse tumor models. Observance of safe ty precautions will help avoid actions that could damage or adversely affect the performance of the ivis spectrum. Ivis spectrum imaging research technology support facility. Bioluminescent imager for measuring and quantifying fluorescent or chemiluminescent luciferinluciferase signal expression in invivo, exvivo or invitro specimens.
Advanced imaging software designed for ivis lumina series optical preclinical in vivo imaging systems. Nearinfrared fluorescent proteins are in high demand for in vivo imaging. Feb 19, 2015 this training video discusses using the ivis platform for in vivo bioluminescence imaging studies to evaluate 2 separate mouse tumor models. For more information about pricing and product details, contact a meditech 7818200 or spectrum medical 8002652331 ext. Ivis spectrum best practices university of florida.
Ivis spectrum 4 multimodality coregistration fluorescence ct coregistration analysis the ivis spectrum is the most advanced in vivo imaging system available on the market today not only can it quantitate and localize 3d fluorescent and bioluminescent sources in vivo, but it can import and automatically coregister. Living image advanced in vivo imaging software designed for the ivis lumina platform simplifies even the most complex image acquisition and analysis of 2d bioluminescent and fluorescent probes. Laboratory equipment perkinelmer caliper ivis spectrum hardware manual. Precise optical and xray overlay allows you to bring your optical signal into anatomical context. An optimized set of high efficiency filters and spectral unmixing algorithms lets you take full advantage of bioluminescent and fluorescent reporters across the blue to.
Our users transition from ivis to lago was seamless. Software images are acquired in a two step process sensitivity is controlled by integration time, fstop, and binning living image controls ivis and provides image analysis tools fluorescence tissue autofluorescence and instrument background can be subtracted. Epiillumination is the standard 2d image that is covered in basic training. Ivis is listed in the worlds largest and most authoritative dictionary database of abbreviations and acronyms the free dictionary. Ivis is a wine production, vineyard management, and quality management system qms. With streamlined interfaces and workflows for longitudinal analysis of 2d data including coregistration with xray in. In vivo bioluminescence imaging on the ivis platform youtube. The ivis spectrum is the newest instrument in the ivis line of products and performs bioluminescent. Ivis software ivis innovative vinology information system. Advanced acquisition and analysis tools for ivis lumina imaging systems. Aura is a comprehensive, feature rich software analysis tool for optical imaging. Ivis spectrum in vivo imaging system university imaging centers. Spectrum two n users guide using the spectrum onscreen help system use the spectrum help system to find further information about using spectrum software to control, set up and adjust your instrument. The living image software as well as microsoft office software is installed on the computer.
Kinetic, please see the living image software users manual specific for the ivis kinetic. Ivis spectrum in vivo imaging system university imaging. Nonhuman primate ivis spectrum imaging system uc davis. Ivis pro identifies and exposes areas where your company is vulnerable to risk and fraud incorporates the fraud triangle to help you evaluate your organizations risk and select scores for rationalization, opportunity, pressure and consequence. The ivis spectrum in vivo imaging system combines 2d optical and 3d optical tomography in one platform. The instrumentation resource facility uses agilient crosslabs ilab software to manage equipment availability and service requests. As a way to fully develop and visualize the technology, ivis software livingimage is capable of making 3dimensional images of the bioluminescent signal figure 4 which allows us to easily identify locations of high viral replication based upon tissue depth and composition. Transillumination and 3d imaging will require an advanced training session, at. This is best achieved with multiple dilutions of the product in a black well plate. It has applications in many fields of research such as oncology, stem cell research, infectious disease. Zarychtawisniewska w, burdzinska a, zagozdzon r, dybowski b, butrym m, gajewski z, et al.
Visit the customer help manual for further guidance using ilabs. We developed four spectrally distinct fluorescent proteins, irfp670, irfp682, irfp702, and irfp720, from bacterial phytochromes. The living image software for automated image acquisition, postprocessing, and data analysis. Living image in vivo software is available for the ivis lumina and ivis spectrum series. The ivis spectrum ct automates the complete workflow from image acquisition and reconstruction to. The ivis spectrum is an optical imager used to facilitate noninvasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals. Living image software ivis spectrum series perkinelmer. Ivis spectrum best practices preparing your experiment before you image any live animals, you should test out your luminescent or fluorescent imaging product in vitro first. Perkinelmer spectrum two n user manual pdf download.
Nearinfrared fluorescent proteins for multicolor in vivo imaging. All ivis modalities can be used, also in combination. Imaging data was acquired through the cornell university biotechnology resource center, with nih s10od025049 funding for the ivis spectrum imaging platform. Ivis information shows the latest news from ivis about the system, training opportunities, tips and tricks and much more. Our assessment process features the ability to enter justification information for each score. The ivis spectrum is the most versatile and advanced in vivo imaging system available on the market today. Perkinelmercaliper for the assistance with the living image software, and p. Many of these show images of the machine and the software. Home page international veterinary information service.
The incredibly sensitive camera combined with high energy trans and epiillumination makes bioluminescence. New anesthesia interface suite from meditech and spectrum medical. The ivis spectrum is equipped wit h a strong bottom illumination fluorescence light source. This will ensure that your signal is detectable before further investments of time and. The rtsf ivis spectrum imaging core at michigan state university is a versatile. The ivis spectrum is the newest instrument in the ivis line of products and performs bioluminescent and epi and trans illumination fluorescent imaging. Ivis filter recommendations f i lter recommendat i on s ivis spectrum, lumina ii and kinetic corporate headquarters 68 elm. The images may be clicked to display as full sized. Ivis spectrum in vivo imager university of california, berkeley.
Saving spectra all spectra in spectrum software are saved to. View profile is available above the information panel and when clicked opens the profile editor where you can update your name, username, password and contact information. Oxygen tank is provided by cam and connected with anesthesia machine and if the. The ivis spectrum is the most versatile and advanced in vivo imaging system available. Equipment ivis lumina xr md anderson cancer center. The ivis spectrum is perkinelmers marketleading preclinical imaging system. Available filter pairs for ivis instrumentation filter recommendations for common fluorescent proteins, dyes and quantum dots using the ivis spectrum and lumina ii or ivis kinetic equipped with spectral unmixing filters. The following page is provided as a handy reference guide to many to the basic functions in the operation of the ivis spectrum luminescence fluorescence imager. Cleaning the ivis spectrum xg18 anesthesia machine operating protocol quick reference operating protocol vaporizer how to fill and drain xic3 isolation chamber use of cleaning pro. Meditech and spectrum medical are interested in working with sites now to arrange deployment of these anesthesia interfaces. If it is necessary to relocate the ivis lumina, contact xenogen technical support.
With streamlined interfaces and workflows for longitudinal analysis of 2d. Ivis filter recommendations ivis spectrum, lumina ii and. Due to the risk of potential damage, it is critical that only a trained technician moves the ivis lumina. Nearinfrared fluorescent proteins for multicolor in vivo.
Like previous ivis systems, it uses xenogens novel patented optical imaging technology to facilitate noninvasive longitudinal monitoring of disease progression, cell trafficking, and gene expression patterns in living animals. If the equipment is used in a ma nner not specified in this manual, the. Advanced acquisition and analysis tools for ivis spectrum imaging systems. Ivis protocols research technology support facility. The ivis spectrum imaging system can be used in both reflectance and transmission modes and includes a highly sensitive backthinned, backilluminated ccd camera, a broad band excitation source with filters for fluorescence measurements, selectable filters for multispectral detection, and software to control data acquisition, for spectral. Living image software also serves as a guide to help inexperienced users through the complex steps associated with quantitative in vivo imaging. Ivis technologies transforming the way organizations engage.
For our experiment well use the ivis spectrum in vivo imaging system uses a backthinned charge coupled device cooled to 90c to achieve maximum sensitivity. To support absolute quantitation, the system measures dark charge during downtime and runs a selfcalibration during initialization. Logout is available in the footer on the home screen. The living image software supports hardware for ivis imaging. Spectral instruments imaging aura imaging software. Use the links below to quickly find your software solution download. Ivis technologies transforming the way organizations. The instrument is equipped with 10 narrow band excitation filters 30nm bandwidth and 18 narrow band emission filters 20nm bandwidth that assist in. Transillumination and 3d imaging will require an advanced training session, at the users request. Ivis spectrum ct for xraybased scanning, additionally. The imaging wizard will help you design imaging settings for fluorescence and bioluminescence protocols.
Ivis spectrum imaging wizard making it simple advanced imaging with confidence living image 3. The ivis lumina is sensitive, scientific equipment and should not be moved by any user unless the system is located on the xws260 workstation. Right click on the link to the file and choose save target as and save the zip file to your desktop. Cs small animal computer tomography leibniz institute on aging. In vivo bioluminescent imaging of mammary tumors using ivis spectrum. For those of you who have previously used our ivis 100, you will find that this new system is truly state of the art in comparison. An optimized set of high efficiency filters and spectral unmixing algorithms lets you take full advantage of bioluminescent and fluorescent reporters across the blue to near infrared wavelength region. In living image software, exposure time, fstop and pixel binning can be optimized based on the expression level of the. Calipers living image software is designed to simplify advanced and complex biological models by intuitively guiding the user in experimental setup and analysis.
In vivo bioluminescent imaging of mammary tumors using ivis. The rtsf ivis spectrum imaging core at michigan state university is a versatile, advanced in vivo bioluminescent and fluorescent preclinical imaging system that combines highthroughput and full tomographic optical imaging in one platform. The ivis website provides free access to original, uptodate publications organized in electronic books each edited by highly qualified editors, proceedings of veterinary meetings, journals, short courses, an international calendar of veterinary events and much more, with the help of private and corporate sponsors. Ivis spectrum user guide booking reservation for the ivis spectrum must be done through the nu core scheduler. The ivis spectrum ct automates the complete workflow from image acquisition and reconstruction to coregistration. Ivis spectrum in vivo imager university of california. Please see the ivis spectrum ct hardware manual part no. Jun 15, 2011 the ivis spectrum is the most versatile and advanced in vivo imaging system available.
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